To change normality, all proportion analysis was in fact transformed just before analyses

To change normality, all proportion analysis was in fact transformed just before analyses

A standard, cumulative directory from get across-fitness is actually determined for each mixture of maternal tree and you will pollen donor, based on adult fresh fruit lay, vegetables germination, and survivorship and you may growth of seedlings. Per kinds, mixed-model data off difference was utilized to assess the consequences out of crossing medication (fixed feeling; which have maternal forest incorporated because a haphazard impression) into part of hand-pollinated plants form mature fresh fruit, costs out-of seed products germination and you will seedling survivorship, seedling dimensions during the step 1 year, and collective fitness. Numerous habits was basically tested playing with ANOVA: (a) plus every treatments, (b) leaving out unbalanced treatments, allowing comparison regarding correspondence terms and conditions, (c) without selfing procedures (because maternal woods was basically mainly or totally care about-incompatible), and you will (d) group most of the contained in this-Sinharaja outcrossing treatments to test the end result out of within- vs. between-tree crossing. The end result out of crossing distance for each parameter are then tested having fun with linear or quadratic regression data, according to the model of the partnership. Finally, each maternal forest, the effects away from nearby-neighbors and you can a lot of time-range mating had been projected by way of indicator from biparental inbreeding despair and you may outbreeding despair, respectively, considering cumulative fitness philosophy.

Syzygium rubicundum

Fruit abortion was heavy for all trees, resulting in low fruit set (range across treatments: 2.0–9.7%; Fig. 2a). The timing of abortion was not discernable across treatments. Self-compatibility was low, but variable, across maternal trees (Fig. 2a). Flowers used for tests of apomixis (N = 360) and autogamy (N = 582) failed to set fruit. All analyses of variance in fruit set revealed a highly significant treatment effect and significant maternal tree effect, but no significant interaction between treatment and maternal tree (Tables 2A and 3A). For all three trees, the percentage of experimental flowers setting mature fruit showed a consistent increase with crossing distance, followed by a severe decline in fruit set with the distant between-forest treatment (Fig. 2a) site de rencontre gratuit pour célibataires polyamoureux. The relationship between crossing distance and fruit set was nearly identical for the three maternal trees and significant with or without the self-pollinated treatment included in the model (quadratic regression model: arcsine square-root [fruit set] = crossing distance [km] + crossing distance 2 ; results without self-pollinated treatment: F2,57 = 8.25, P < 0.0007, R 2 = 0.47). Peak mean fruit set occurred at a crossing distance of 1–2 km (distant within-forest treatment) and was 1.7–4.7 times greater than mean fruit set rates for other hand-pollination treatments, averaged across maternal trees. Mean fruit set rate for the distant within-forest treatment was significantly greater than those for all treatments except distant-neighbor and open-pollinated, but consistently exceeded fruit set of open-pollinated flowers (Fig. 2a).

Shorea cordifolia

Fruit set was also low for Sh. cordifolia (range across treatments: 0–5.3%; Fig. 2b). Again, the timing of fruit abortion was not discernable among treatments. Selfed and distant between-forest treatments resulted in 0% and <1% fruit set, respectively. Fruit set from the intermediate-distance cross-pollinations varied across maternal trees, but with one exception (nearest-neighbor treatment at Tree number 1) indicated optimal fruit set at an outcrossing range of ?2 km (distant neighbor treatment; Fig. 2b). All analyses of variance in fruit set revealed a highly significant treatment effect, but no maternal tree effect (Tables 2B and 3B). The relationship between crossing distance and fruit set was significant only when the selfed treatment was excluded (quadratic regression model: arcsin square root [fruit set] = crossing distance [km] + crossing distance 2 ; F2,57 = 5.71, P < 0.006, R 2 = 0.41). At each maternal tree, fruit set rate for open-pollinated flowers was greater than that for all hand-cross treatments, suggesting that some aspect of the hand-pollination procedure (e.g., flower handling, bagging) caused reduced fruit set in Sh. cordifolia.

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